Construct: shRNA TRCN0000178804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001034832.1-596s1c1
DNA Barcode:
CCAAGTACCTTGGAGAAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SSX4B (548313)
Gene Description:
SSX family member 4B
Transcript:
RefSeq NM_001034832.1 (NON-CURRENT)
Match location:
Position 596 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGTACCTTGGAGAAGATT
Hairpin Sequence:
5'-CCGG-CCAAGTACCTTGGAGAAGATT-CTCGAG-AATCTTCTCCAAGGTACTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGTACCTTGGAGAAGATTCTCGAGAATCTTCTCCAAGGTACTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGTACCTTGGAGAAGATTCTCGAGAATCTTCTCCAAGGTACTTGG-3'

Other clones with same target sequence:

(none)