Construct: shRNA TRCN0000178817

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027789.2-757s1c1
DNA Barcode:
CCCTCGACATTTATTCTGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spaar (71406)
Gene Description:
small regulatory polypeptide of amino acid response
Transcript:
RefSeq NM_027789.2 (NON-CURRENT)
Match location:
Position 757 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178817)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCGACATTTATTCTGCTA
Hairpin Sequence:
5'-CCGG-CCCTCGACATTTATTCTGCTA-CTCGAG-TAGCAGAATAAATGTCGAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCGACATTTATTCTGCTACTCGAGTAGCAGAATAAATGTCGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCGACATTTATTCTGCTACTCGAGTAGCAGAATAAATGTCGAGGG-3'

Other clones with same target sequence:

(none)