Construct: shRNA TRCN0000178848

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012455.1-1558s1c1
DNA Barcode:
CAGCCAAGTTCCTTGAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSD4 (23550)
Gene Description:
pleckstrin and Sec7 domain containing 4
Transcript:
RefSeq NM_012455.1 (NON-CURRENT)
Match location:
Position 1558 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178848)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCCAAGTTCCTTGAAGAAA
Hairpin Sequence:
5'-CCGG-CAGCCAAGTTCCTTGAAGAAA-CTCGAG-TTTCTTCAAGGAACTTGGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCCAAGTTCCTTGAAGAAACTCGAGTTTCTTCAAGGAACTTGGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCCAAGTTCCTTGAAGAAACTCGAGTTTCTTCAAGGAACTTGGCTG-3'

Other clones with same target sequence:

(none)