Construct: shRNA TRCN0000178920

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006327.2-349s1c1
DNA Barcode:
CCTTCTTTACGATTGGAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TIMM23 (100287932)
Gene Description:
translocase of inner mitochondrial membrane 23
Transcript:
RefSeq NM_006327.2 (NON-CURRENT)
Match location:
Position 349 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000178920)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTTTACGATTGGAGGAT
Hairpin Sequence:
5'-CCGG-CCTTCTTTACGATTGGAGGAT-CTCGAG-ATCCTCCAATCGTAAAGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTTTACGATTGGAGGATCTCGAGATCCTCCAATCGTAAAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTTTACGATTGGAGGATCTCGAGATCCTCCAATCGTAAAGAAGG-3'

Other clones with same target sequence:

(none)