Construct: shRNA TRCN0000179009

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003955.2-1746s1c1
DNA Barcode:
CCATGCTAAGCACTAACCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab11fip5 (52055)
Gene Description:
RAB11 family interacting protein 5 (class I)
Transcript:
RefSeq NM_001003955.2 (CURRENT)
Match location:
Position 1746 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179009)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCTAAGCACTAACCTTT
Hairpin Sequence:
5'-CCGG-CCATGCTAAGCACTAACCTTT-CTCGAG-AAAGGTTAGTGCTTAGCATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCTAAGCACTAACCTTTCTCGAGAAAGGTTAGTGCTTAGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCTAAGCACTAACCTTTCTCGAGAAAGGTTAGTGCTTAGCATGG-3'

Other clones with same target sequence:

(none)