Construct: shRNA TRCN0000179050

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025416.1-1051s1c1
DNA Barcode:
CCACAGATAACTCAGCAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Them5 (66198)
Gene Description:
thioesterase superfamily member 5
Transcript:
RefSeq NM_025416.1 (NON-CURRENT)
Match location:
Position 1051 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179050)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGATAACTCAGCAGTTA
Hairpin Sequence:
5'-CCGG-CCACAGATAACTCAGCAGTTA-CTCGAG-TAACTGCTGAGTTATCTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGATAACTCAGCAGTTACTCGAGTAACTGCTGAGTTATCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGATAACTCAGCAGTTACTCGAGTAACTGCTGAGTTATCTGTGG-3'

Other clones with same target sequence:

(none)