Construct: shRNA TRCN0000179092

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005745.5-856s1c1
DNA Barcode:
CATGGACAAGAAGGAAGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCAP31 (10134)
Gene Description:
B cell receptor associated protein 31
Transcript:
RefSeq NM_005745.5 (NON-CURRENT)
Match location:
Position 856 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179092)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGACAAGAAGGAAGAGTA
Hairpin Sequence:
5'-CCGG-CATGGACAAGAAGGAAGAGTA-CTCGAG-TACTCTTCCTTCTTGTCCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGACAAGAAGGAAGAGTACTCGAGTACTCTTCCTTCTTGTCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGACAAGAAGGAAGAGTACTCGAGTACTCTTCCTTCTTGTCCATG-3'

Other clones with same target sequence:

(none)