Construct: shRNA TRCN0000179162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027990.1-723s1c1
DNA Barcode:
GCAGGACACACAGTATTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lypd6b (71897)
Gene Description:
LY6/PLAUR domain containing 6B
Transcript:
RefSeq NM_027990.1 (NON-CURRENT)
Match location:
Position 723 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGACACACAGTATTGTTT
Hairpin Sequence:
5'-CCGG-GCAGGACACACAGTATTGTTT-CTCGAG-AAACAATACTGTGTGTCCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGACACACAGTATTGTTTCTCGAGAAACAATACTGTGTGTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGACACACAGTATTGTTTCTCGAGAAACAATACTGTGTGTCCTGC-3'

Other clones with same target sequence:

(none)