Construct: shRNA TRCN0000179268

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026442.2-87s1c1
DNA Barcode:
GAAAGATGTTCTGATCCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cmc1 (67899)
Gene Description:
COX assembly mitochondrial protein 1
Transcript:
RefSeq NM_026442.2 (NON-CURRENT)
Match location:
Position 87 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179268)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGATGTTCTGATCCCAAA
Hairpin Sequence:
5'-CCGG-GAAAGATGTTCTGATCCCAAA-CTCGAG-TTTGGGATCAGAACATCTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGATGTTCTGATCCCAAACTCGAGTTTGGGATCAGAACATCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGATGTTCTGATCCCAAACTCGAGTTTGGGATCAGAACATCTTTC-3'

Other clones with same target sequence:

(none)