Construct: shRNA TRCN0000179272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030241.2-220s1c1
DNA Barcode:
GCAGTCAAAGATCTATGCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kmt5a (67956)
Gene Description:
lysine methyltransferase 5A
Transcript:
RefSeq NM_030241.2 (NON-CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCAAAGATCTATGCCTA
Hairpin Sequence:
5'-CCGG-GCAGTCAAAGATCTATGCCTA-CTCGAG-TAGGCATAGATCTTTGACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCAAAGATCTATGCCTACTCGAGTAGGCATAGATCTTTGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCAAAGATCTATGCCTACTCGAGTAGGCATAGATCTTTGACTGC-3'

Other clones with same target sequence:

(none)