Construct: shRNA TRCN0000179290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178149.2-1138s1c1
DNA Barcode:
GAAAGAGCAACATGAGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3ip1 (216505)
Gene Description:
phosphoinositide-3-kinase interacting protein 1
Transcript:
RefSeq NM_178149.2 (NON-CURRENT)
Match location:
Position 1138 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGAGCAACATGAGAAGAA
Hairpin Sequence:
5'-CCGG-GAAAGAGCAACATGAGAAGAA-CTCGAG-TTCTTCTCATGTTGCTCTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGAGCAACATGAGAAGAACTCGAGTTCTTCTCATGTTGCTCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGAGCAACATGAGAAGAACTCGAGTTCTTCTCATGTTGCTCTTTC-3'

Other clones with same target sequence:

(none)