Construct: shRNA TRCN0000179315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028887.2-1212s1c1
DNA Barcode:
GCAGGTGATGTAATACGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smchd1 (74355)
Gene Description:
SMC hinge domain containing 1
Transcript:
RefSeq NM_028887.2 (NON-CURRENT)
Match location:
Position 1212 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGTGATGTAATACGCAAT
Hairpin Sequence:
5'-CCGG-GCAGGTGATGTAATACGCAAT-CTCGAG-ATTGCGTATTACATCACCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGTGATGTAATACGCAATCTCGAGATTGCGTATTACATCACCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGTGATGTAATACGCAATCTCGAGATTGCGTATTACATCACCTGC-3'

Other clones with same target sequence:

(none)