Construct: shRNA TRCN0000179339

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020651.2-4202s1c1
DNA Barcode:
GCACTGTGCATATTGCTTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PELI1 (57162)
Gene Description:
pellino E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_020651.2 (NON-CURRENT)
Match location:
Position 4202 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179339)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGTGCATATTGCTTGTA
Hairpin Sequence:
5'-CCGG-GCACTGTGCATATTGCTTGTA-CTCGAG-TACAAGCAATATGCACAGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGTGCATATTGCTTGTACTCGAGTACAAGCAATATGCACAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGTGCATATTGCTTGTACTCGAGTACAAGCAATATGCACAGTGC-3'

Other clones with same target sequence:

(none)