Construct: shRNA TRCN0000179438

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017995.1-231s1c1
DNA Barcode:
GCCCAACAAGCATTATGTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3PXD2B (285590)
Gene Description:
SH3 and PX domains 2B
Transcript:
RefSeq NM_001017995.1 (NON-CURRENT)
Match location:
Position 231 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179438)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAACAAGCATTATGTCTA
Hairpin Sequence:
5'-CCGG-GCCCAACAAGCATTATGTCTA-CTCGAG-TAGACATAATGCTTGTTGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAACAAGCATTATGTCTACTCGAGTAGACATAATGCTTGTTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAACAAGCATTATGTCTACTCGAGTAGACATAATGCTTGTTGGGC-3'

Other clones with same target sequence:

(none)