Construct: shRNA TRCN0000179454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183252.1-378s1c1
DNA Barcode:
GCCTTCATCAAGTGGTATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pantr1 (66297)
Gene Description:
POU domain, class 3, transcription factor 3 adjacent noncoding transcript 1
Transcript:
RefSeq NM_183252.1 (NON-CURRENT)
Match location:
Position 378 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCATCAAGTGGTATGAA
Hairpin Sequence:
5'-CCGG-GCCTTCATCAAGTGGTATGAA-CTCGAG-TTCATACCACTTGATGAAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCATCAAGTGGTATGAACTCGAGTTCATACCACTTGATGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCATCAAGTGGTATGAACTCGAGTTCATACCACTTGATGAAGGC-3'

Other clones with same target sequence:

(none)