Construct: shRNA TRCN0000179509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173746.2-1301s1c1
DNA Barcode:
GCCATTTAATTGTGCAGGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Triqk (208820)
Gene Description:
triple QxxK/R motif containing
Transcript:
RefSeq NM_173746.2 (NON-CURRENT)
Match location:
Position 1301 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTTAATTGTGCAGGGTT
Hairpin Sequence:
5'-CCGG-GCCATTTAATTGTGCAGGGTT-CTCGAG-AACCCTGCACAATTAAATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTTAATTGTGCAGGGTTCTCGAGAACCCTGCACAATTAAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTTAATTGTGCAGGGTTCTCGAGAACCCTGCACAATTAAATGGC-3'

Other clones with same target sequence:

(none)