Construct: shRNA TRCN0000179526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007458.1-1127s1c1
DNA Barcode:
GCTATCCTCTTTGAGACCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap2a1 (11771)
Gene Description:
adaptor-related protein complex 2, alpha 1 subunit
Transcript:
RefSeq NM_007458.1 (NON-CURRENT)
Match location:
Position 1127 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATCCTCTTTGAGACCATT
Hairpin Sequence:
5'-CCGG-GCTATCCTCTTTGAGACCATT-CTCGAG-AATGGTCTCAAAGAGGATAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATCCTCTTTGAGACCATTCTCGAGAATGGTCTCAAAGAGGATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATCCTCTTTGAGACCATTCTCGAGAATGGTCTCAAAGAGGATAGC-3'

Other clones with same target sequence:

  1. TRCN0000277543