Construct: shRNA TRCN0000179604

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172433.1-429s1c1
DNA Barcode:
GTTAACTACACCTGTCCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D730001G18Rik (78725)
Gene Description:
RIKEN cDNA D730001G18 gene
Transcript:
RefSeq NM_172433.1 (NON-CURRENT)
Match location:
Position 429 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179604)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTAACTACACCTGTCCCAAA
Hairpin Sequence:
5'-CCGG-GTTAACTACACCTGTCCCAAA-CTCGAG-TTTGGGACAGGTGTAGTTAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTAACTACACCTGTCCCAAACTCGAGTTTGGGACAGGTGTAGTTAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTAACTACACCTGTCCCAAACTCGAGTTTGGGACAGGTGTAGTTAAC-3'

Other clones with same target sequence:

(none)