Construct: shRNA TRCN0000179614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033172.1-1037s1c1
DNA Barcode:
GCCCATTCGATGTCTGATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab11fip2 (74998)
Gene Description:
RAB11 family interacting protein 2 (class I)
Transcript:
RefSeq NM_001033172.1 (NON-CURRENT)
Match location:
Position 1037 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATTCGATGTCTGATTTA
Hairpin Sequence:
5'-CCGG-GCCCATTCGATGTCTGATTTA-CTCGAG-TAAATCAGACATCGAATGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATTCGATGTCTGATTTACTCGAGTAAATCAGACATCGAATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATTCGATGTCTGATTTACTCGAGTAAATCAGACATCGAATGGGC-3'

Other clones with same target sequence:

(none)