Construct: shRNA TRCN0000179619

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021548.3-246s1c1
DNA Barcode:
GTAACTAGTCCAGAGAAAGCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arpp19 (59046)
Gene Description:
cAMP-regulated phosphoprotein 19
Transcript:
RefSeq NM_021548.3 (NON-CURRENT)
Match location:
Position 246 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179619)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAACTAGTCCAGAGAAAGCT
Hairpin Sequence:
5'-CCGG-GTAACTAGTCCAGAGAAAGCT-CTCGAG-AGCTTTCTCTGGACTAGTTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAACTAGTCCAGAGAAAGCTCTCGAGAGCTTTCTCTGGACTAGTTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAACTAGTCCAGAGAAAGCTCTCGAGAGCTTTCTCTGGACTAGTTAC-3'

Other clones with same target sequence:

(none)