Construct: shRNA TRCN0000179713

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017982.1-1468s1c1
DNA Barcode:
GCAGTGAGAAAGGAAAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUSD4 (55061)
Gene Description:
sushi domain containing 4
Transcript:
RefSeq NM_017982.1 (NON-CURRENT)
Match location:
Position 1468 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179713)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGAGAAAGGAAAGGAAA
Hairpin Sequence:
5'-CCGG-GCAGTGAGAAAGGAAAGGAAA-CTCGAG-TTTCCTTTCCTTTCTCACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGAGAAAGGAAAGGAAACTCGAGTTTCCTTTCCTTTCTCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGAGAAAGGAAAGGAAACTCGAGTTTCCTTTCCTTTCTCACTGC-3'

Other clones with same target sequence:

(none)