Construct: shRNA TRCN0000179778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030679.1-2251s1c1
DNA Barcode:
GCAGACTTCAAGCAGAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myh1 (17879)
Gene Description:
myosin, heavy polypeptide 1, skeletal muscle, adult
Transcript:
RefSeq NM_030679.1 (CURRENT)
Match location:
Position 2251 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGACTTCAAGCAGAGATAT
Hairpin Sequence:
5'-CCGG-GCAGACTTCAAGCAGAGATAT-CTCGAG-ATATCTCTGCTTGAAGTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGACTTCAAGCAGAGATATCTCGAGATATCTCTGCTTGAAGTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGACTTCAAGCAGAGATATCTCGAGATATCTCTGCTTGAAGTCTGC-3'

Other clones with same target sequence:

(none)