Construct: shRNA TRCN0000179864

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024510.2-1750s1c1
DNA Barcode:
CAGCAAGATCCTGTCTTTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYBC1 (79415)
Gene Description:
cytochrome b-245 chaperone 1
Transcript:
RefSeq NM_024510.2 (NON-CURRENT)
Match location:
Position 1750 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179864)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCAAGATCCTGTCTTTGTA
Hairpin Sequence:
5'-CCGG-CAGCAAGATCCTGTCTTTGTA-CTCGAG-TACAAAGACAGGATCTTGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCAAGATCCTGTCTTTGTACTCGAGTACAAAGACAGGATCTTGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCAAGATCCTGTCTTTGTACTCGAGTACAAAGACAGGATCTTGCTG-3'

Other clones with same target sequence:

(none)