Construct: shRNA TRCN0000179866

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027347.2-2833s1c1
DNA Barcode:
CCACAAGAAATATCCCGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Med23 (70208)
Gene Description:
mediator complex subunit 23
Transcript:
RefSeq NM_027347.2 (NON-CURRENT)
Match location:
Position 2833 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179866)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAAGAAATATCCCGAGAA
Hairpin Sequence:
5'-CCGG-CCACAAGAAATATCCCGAGAA-CTCGAG-TTCTCGGGATATTTCTTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAAGAAATATCCCGAGAACTCGAGTTCTCGGGATATTTCTTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAAGAAATATCCCGAGAACTCGAGTTCTCGGGATATTTCTTGTGG-3'

Other clones with same target sequence:

  1. TRCN0000341854