Construct: shRNA TRCN0000179949

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029306.1-492s1c1
DNA Barcode:
CCCAGGCTTGTTTCTCAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1700012B09Rik (69325)
Gene Description:
RIKEN cDNA 1700012B09 gene
Transcript:
RefSeq NM_029306.1 (NON-CURRENT)
Match location:
Position 492 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179949)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGCTTGTTTCTCAATTA
Hairpin Sequence:
5'-CCGG-CCCAGGCTTGTTTCTCAATTA-CTCGAG-TAATTGAGAAACAAGCCTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGCTTGTTTCTCAATTACTCGAGTAATTGAGAAACAAGCCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGCTTGTTTCTCAATTACTCGAGTAATTGAGAAACAAGCCTGGG-3'

Other clones with same target sequence:

(none)