Construct: shRNA TRCN0000179969

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001025616.1-2765s1c1
DNA Barcode:
CCTGGATGTACAGAAGTCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP24 (83478)
Gene Description:
Rho GTPase activating protein 24
Transcript:
RefSeq NM_001025616.1 (NON-CURRENT)
Match location:
Position 2765 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179969)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGATGTACAGAAGTCTAA
Hairpin Sequence:
5'-CCGG-CCTGGATGTACAGAAGTCTAA-CTCGAG-TTAGACTTCTGTACATCCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGATGTACAGAAGTCTAACTCGAGTTAGACTTCTGTACATCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGATGTACAGAAGTCTAACTCGAGTTAGACTTCTGTACATCCAGG-3'

Other clones with same target sequence:

(none)