Construct: shRNA TRCN0000179981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026411.1-156s1c1
DNA Barcode:
CGTCACACAAACTTTGTGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1700021F05Rik (67851)
Gene Description:
RIKEN cDNA 1700021F05 gene
Transcript:
RefSeq NM_026411.1 (CURRENT)
Match location:
Position 156 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000179981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCACACAAACTTTGTGCTT
Hairpin Sequence:
5'-CCGG-CGTCACACAAACTTTGTGCTT-CTCGAG-AAGCACAAAGTTTGTGTGACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCACACAAACTTTGTGCTTCTCGAGAAGCACAAAGTTTGTGTGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCACACAAACTTTGTGCTTCTCGAGAAGCACAAAGTTTGTGTGACG-3'

Other clones with same target sequence:

(none)