Construct: shRNA TRCN0000180016

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027590.1-1680s1c1
DNA Barcode:
CCAAAGTAAAGCCCAAGTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ints10 (70885)
Gene Description:
integrator complex subunit 10
Transcript:
RefSeq NM_027590.1 (NON-CURRENT)
Match location:
Position 1680 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180016)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGTAAAGCCCAAGTGTA
Hairpin Sequence:
5'-CCGG-CCAAAGTAAAGCCCAAGTGTA-CTCGAG-TACACTTGGGCTTTACTTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGTAAAGCCCAAGTGTACTCGAGTACACTTGGGCTTTACTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGTAAAGCCCAAGTGTACTCGAGTACACTTGGGCTTTACTTTGG-3'

Other clones with same target sequence:

(none)