Construct: shRNA TRCN0000180072

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026001.1-1047s1c1
DNA Barcode:
CGCTGAGAATCAAGGTACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnaseh2b (67153)
Gene Description:
ribonuclease H2, subunit B
Transcript:
RefSeq NM_026001.1 (NON-CURRENT)
Match location:
Position 1047 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180072)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGAGAATCAAGGTACTTT
Hairpin Sequence:
5'-CCGG-CGCTGAGAATCAAGGTACTTT-CTCGAG-AAAGTACCTTGATTCTCAGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGAGAATCAAGGTACTTTCTCGAGAAAGTACCTTGATTCTCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGAGAATCAAGGTACTTTCTCGAGAAAGTACCTTGATTCTCAGCG-3'

Other clones with same target sequence:

(none)