Construct: shRNA TRCN0000180101

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020335.1-930s1c1
DNA Barcode:
CTTCATCTCTGTCGCCTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VANGL2 (57216)
Gene Description:
VANGL planar cell polarity protein 2
Transcript:
RefSeq NM_020335.1 (NON-CURRENT)
Match location:
Position 930 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180101)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCATCTCTGTCGCCTTCAA
Hairpin Sequence:
5'-CCGG-CTTCATCTCTGTCGCCTTCAA-CTCGAG-TTGAAGGCGACAGAGATGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCATCTCTGTCGCCTTCAACTCGAGTTGAAGGCGACAGAGATGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCATCTCTGTCGCCTTCAACTCGAGTTGAAGGCGACAGAGATGAAG-3'

Other clones with same target sequence:

(none)