Construct: shRNA TRCN0000180184

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024672.1-185s1c1
DNA Barcode:
CGGTTCGCCATGATATACAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Methig1 (554292)
Gene Description:
methyltransferase hypoxia inducible domain containing 1
Transcript:
RefSeq NM_001024672.1 (NON-CURRENT)
Match location:
Position 185 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180184)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTCGCCATGATATACAAT
Hairpin Sequence:
5'-CCGG-CGGTTCGCCATGATATACAAT-CTCGAG-ATTGTATATCATGGCGAACCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTCGCCATGATATACAATCTCGAGATTGTATATCATGGCGAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTCGCCATGATATACAATCTCGAGATTGTATATCATGGCGAACCG-3'

Other clones with same target sequence:

  1. TRCN0000097571