Construct: shRNA TRCN0000180221

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026001.1-957s1c1
DNA Barcode:
CCAGTGAAGCAGAAACAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnaseh2b (67153)
Gene Description:
ribonuclease H2, subunit B
Transcript:
RefSeq NM_026001.1 (NON-CURRENT)
Match location:
Position 957 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180221)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGAAGCAGAAACAGAAA
Hairpin Sequence:
5'-CCGG-CCAGTGAAGCAGAAACAGAAA-CTCGAG-TTTCTGTTTCTGCTTCACTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGAAGCAGAAACAGAAACTCGAGTTTCTGTTTCTGCTTCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGAAGCAGAAACAGAAACTCGAGTTTCTGTTTCTGCTTCACTGG-3'

Other clones with same target sequence:

(none)