Construct: shRNA TRCN0000180241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003724.1-1680s1c1
DNA Barcode:
CCACAACAAGTCCTTTGCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JRK (8629)
Gene Description:
Jrk helix-turn-helix protein
Transcript:
RefSeq NM_003724.1 (NON-CURRENT)
Match location:
Position 1680 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAACAAGTCCTTTGCACA
Hairpin Sequence:
5'-CCGG-CCACAACAAGTCCTTTGCACA-CTCGAG-TGTGCAAAGGACTTGTTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAACAAGTCCTTTGCACACTCGAGTGTGCAAAGGACTTGTTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAACAAGTCCTTTGCACACTCGAGTGTGCAAAGGACTTGTTGTGG-3'

Other clones with same target sequence:

(none)