Construct: shRNA TRCN0000180256

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178745.3-1265s1c1
DNA Barcode:
CGGACATTGATGGATGACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem229b (268567)
Gene Description:
transmembrane protein 229B
Transcript:
RefSeq NM_178745.3 (NON-CURRENT)
Match location:
Position 1265 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180256)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACATTGATGGATGACTTT
Hairpin Sequence:
5'-CCGG-CGGACATTGATGGATGACTTT-CTCGAG-AAAGTCATCCATCAATGTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACATTGATGGATGACTTTCTCGAGAAAGTCATCCATCAATGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACATTGATGGATGACTTTCTCGAGAAAGTCATCCATCAATGTCCG-3'

Other clones with same target sequence:

(none)