Construct: shRNA TRCN0000180274

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024333.1-137s1c1
DNA Barcode:
CCTGAAACAGATGCTGCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FSD1 (79187)
Gene Description:
fibronectin type III and SPRY domain containing 1
Transcript:
RefSeq NM_024333.1 (NON-CURRENT)
Match location:
Position 137 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180274)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAACAGATGCTGCTGAA
Hairpin Sequence:
5'-CCGG-CCTGAAACAGATGCTGCTGAA-CTCGAG-TTCAGCAGCATCTGTTTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAACAGATGCTGCTGAACTCGAGTTCAGCAGCATCTGTTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAACAGATGCTGCTGAACTCGAGTTCAGCAGCATCTGTTTCAGG-3'

Other clones with same target sequence:

(none)