Construct: shRNA TRCN0000180280

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152550.1-685s1c1
DNA Barcode:
CGGAGACAGCTTGATGAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3RF2 (153769)
Gene Description:
SH3 domain containing ring finger 2
Transcript:
RefSeq NM_152550.1 (NON-CURRENT)
Match location:
Position 685 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180280)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGACAGCTTGATGAGAAT
Hairpin Sequence:
5'-CCGG-CGGAGACAGCTTGATGAGAAT-CTCGAG-ATTCTCATCAAGCTGTCTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGACAGCTTGATGAGAATCTCGAGATTCTCATCAAGCTGTCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGACAGCTTGATGAGAATCTCGAGATTCTCATCAAGCTGTCTCCG-3'

Other clones with same target sequence:

(none)