Construct: shRNA TRCN0000180314

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001007584.1-1110s1c1
DNA Barcode:
CCTGTGTGCATGTTTGTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm11541 (432589)
Gene Description:
predicted gene 11541
Transcript:
RefSeq NM_001007584.1 (NON-CURRENT)
Match location:
Position 1110 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180314)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGTGCATGTTTGTGTAT
Hairpin Sequence:
5'-CCGG-CCTGTGTGCATGTTTGTGTAT-CTCGAG-ATACACAAACATGCACACAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGTGCATGTTTGTGTATCTCGAGATACACAAACATGCACACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGTGCATGTTTGTGTATCTCGAGATACACAAACATGCACACAGG-3'

Other clones with same target sequence:

(none)