Construct: shRNA TRCN0000180399

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207264.2-512s1c1
DNA Barcode:
GTTCTAGCAAACCAGGTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
BC052040 (399568)
Gene Description:
cDNA sequence BC052040
Transcript:
RefSeq NM_207264.2 (NON-CURRENT)
Match location:
Position 512 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180399)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCTAGCAAACCAGGTCTAT
Hairpin Sequence:
5'-CCGG-GTTCTAGCAAACCAGGTCTAT-CTCGAG-ATAGACCTGGTTTGCTAGAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCTAGCAAACCAGGTCTATCTCGAGATAGACCTGGTTTGCTAGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCTAGCAAACCAGGTCTATCTCGAGATAGACCTGGTTTGCTAGAAC-3'

Other clones with same target sequence:

(none)