Construct: shRNA TRCN0000180427

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174875.3-1312s1c1
DNA Barcode:
GCCTGATATGCAGATAGCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atg4a (666468)
Gene Description:
autophagy related 4A, cysteine peptidase
Transcript:
RefSeq NM_174875.3 (CURRENT)
Match location:
Position 1312 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180427)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGATATGCAGATAGCATA
Hairpin Sequence:
5'-CCGG-GCCTGATATGCAGATAGCATA-CTCGAG-TATGCTATCTGCATATCAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGATATGCAGATAGCATACTCGAGTATGCTATCTGCATATCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGATATGCAGATAGCATACTCGAGTATGCTATCTGCATATCAGGC-3'

Other clones with same target sequence:

(none)