Construct: shRNA TRCN0000180440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037224.1-260s1c1
DNA Barcode:
GTCGTTTCTGTCCACAGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC145757 (145757)
Gene Description:
uncharacterized LOC145757
Transcript:
RefSeq NM_001037224.1 (NON-CURRENT)
Match location:
Position 260 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGTTTCTGTCCACAGTCAT
Hairpin Sequence:
5'-CCGG-GTCGTTTCTGTCCACAGTCAT-CTCGAG-ATGACTGTGGACAGAAACGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGTTTCTGTCCACAGTCATCTCGAGATGACTGTGGACAGAAACGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGTTTCTGTCCACAGTCATCTCGAGATGACTGTGGACAGAAACGAC-3'

Other clones with same target sequence:

(none)