Construct: shRNA TRCN0000180564

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144653.3-1002s1c1
DNA Barcode:
GTACGGCCAGATGTACATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NACC2 (138151)
Gene Description:
NACC family member 2
Transcript:
RefSeq NM_144653.3 (NON-CURRENT)
Match location:
Position 1002 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180564)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACGGCCAGATGTACATCAA
Hairpin Sequence:
5'-CCGG-GTACGGCCAGATGTACATCAA-CTCGAG-TTGATGTACATCTGGCCGTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACGGCCAGATGTACATCAACTCGAGTTGATGTACATCTGGCCGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACGGCCAGATGTACATCAACTCGAGTTGATGTACATCTGGCCGTAC-3'

Other clones with same target sequence:

(none)