Construct: shRNA TRCN0000180597

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026086.1-719s1c1
DNA Barcode:
GCTAGAATTACCTGCTCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nanp (67311)
Gene Description:
N-acetylneuraminic acid phosphatase
Transcript:
RefSeq NM_026086.1 (NON-CURRENT)
Match location:
Position 719 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180597)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGAATTACCTGCTCTCTT
Hairpin Sequence:
5'-CCGG-GCTAGAATTACCTGCTCTCTT-CTCGAG-AAGAGAGCAGGTAATTCTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGAATTACCTGCTCTCTTCTCGAGAAGAGAGCAGGTAATTCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGAATTACCTGCTCTCTTCTCGAGAAGAGAGCAGGTAATTCTAGC-3'

Other clones with same target sequence:

(none)