Construct: shRNA TRCN0000180617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013559.1-343s1c1
DNA Barcode:
GAGATTTCATGGCAGAGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hsph1 (15505)
Gene Description:
heat shock 105kDa/110kDa protein 1
Transcript:
RefSeq NM_013559.1 (NON-CURRENT)
Match location:
Position 343 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATTTCATGGCAGAGCATT
Hairpin Sequence:
5'-CCGG-GAGATTTCATGGCAGAGCATT-CTCGAG-AATGCTCTGCCATGAAATCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATTTCATGGCAGAGCATTCTCGAGAATGCTCTGCCATGAAATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATTTCATGGCAGAGCATTCTCGAGAATGCTCTGCCATGAAATCTC-3'

Other clones with same target sequence:

(none)