Construct: shRNA TRCN0000180645

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025935.1-309s1c1
DNA Barcode:
CAAAGACCAGTACCATGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbc1d7 (67046)
Gene Description:
TBC1 domain family, member 7
Transcript:
RefSeq NM_025935.1 (NON-CURRENT)
Match location:
Position 309 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180645)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGACCAGTACCATGACAT
Hairpin Sequence:
5'-CCGG-CAAAGACCAGTACCATGACAT-CTCGAG-ATGTCATGGTACTGGTCTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGACCAGTACCATGACATCTCGAGATGTCATGGTACTGGTCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGACCAGTACCATGACATCTCGAGATGTCATGGTACTGGTCTTTG-3'

Other clones with same target sequence:

(none)