Construct: shRNA TRCN0000180674

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015232.1-493s1c1
DNA Barcode:
GCTCAGCAAGAACTTCGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IQSEC3 (440073)
Gene Description:
IQ motif and Sec7 domain ArfGEF 3
Transcript:
RefSeq NM_015232.1 (CURRENT)
Match location:
Position 493 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180674)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAGCAAGAACTTCGAGAA
Hairpin Sequence:
5'-CCGG-GCTCAGCAAGAACTTCGAGAA-CTCGAG-TTCTCGAAGTTCTTGCTGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAGCAAGAACTTCGAGAACTCGAGTTCTCGAAGTTCTTGCTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAGCAAGAACTTCGAGAACTCGAGTTCTCGAAGTTCTTGCTGAGC-3'

Other clones with same target sequence:

(none)