Construct: shRNA TRCN0000180692

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173416.1-1896s1c1
DNA Barcode:
GCTCCATATGTTCACCTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdcp (238037)
Gene Description:
WD repeat and coiled coil containing
Transcript:
RefSeq NM_173416.1 (NON-CURRENT)
Match location:
Position 1896 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180692)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCATATGTTCACCTCATT
Hairpin Sequence:
5'-CCGG-GCTCCATATGTTCACCTCATT-CTCGAG-AATGAGGTGAACATATGGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCATATGTTCACCTCATTCTCGAGAATGAGGTGAACATATGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCATATGTTCACCTCATTCTCGAGAATGAGGTGAACATATGGAGC-3'

Other clones with same target sequence:

(none)