Construct: shRNA TRCN0000180723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013260.4-226s1c1
DNA Barcode:
GACAGTCGGAAGATGACGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SAP30BP (29115)
Gene Description:
SAP30 binding protein
Transcript:
RefSeq NM_013260.4 (NON-CURRENT)
Match location:
Position 226 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAGTCGGAAGATGACGATT
Hairpin Sequence:
5'-CCGG-GACAGTCGGAAGATGACGATT-CTCGAG-AATCGTCATCTTCCGACTGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAGTCGGAAGATGACGATTCTCGAGAATCGTCATCTTCCGACTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAGTCGGAAGATGACGATTCTCGAGAATCGTCATCTTCCGACTGTC-3'

Other clones with same target sequence:

  1. TRCN0000280944