Construct: shRNA TRCN0000180743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172843.2-2597s1c1
DNA Barcode:
GCTGAGTTTCACAGAGAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tor1aip2 (240832)
Gene Description:
torsin A interacting protein 2
Transcript:
RefSeq NM_172843.2 (NON-CURRENT)
Match location:
Position 2597 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGTTTCACAGAGAAGTT
Hairpin Sequence:
5'-CCGG-GCTGAGTTTCACAGAGAAGTT-CTCGAG-AACTTCTCTGTGAAACTCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGTTTCACAGAGAAGTTCTCGAGAACTTCTCTGTGAAACTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGTTTCACAGAGAAGTTCTCGAGAACTTCTCTGTGAAACTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000277370