Construct: shRNA TRCN0000180772

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181612.2-172s1c1
DNA Barcode:
GCCGTGAAGGTTATGGATTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRTAP19-6 (337973)
Gene Description:
keratin associated protein 19-6
Transcript:
RefSeq NM_181612.2 (NON-CURRENT)
Match location:
Position 172 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180772)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTGAAGGTTATGGATTCT
Hairpin Sequence:
5'-CCGG-GCCGTGAAGGTTATGGATTCT-CTCGAG-AGAATCCATAACCTTCACGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTGAAGGTTATGGATTCTCTCGAGAGAATCCATAACCTTCACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTGAAGGTTATGGATTCTCTCGAGAGAATCCATAACCTTCACGGC-3'

Other clones with same target sequence:

  1. TRCN0000245480