Construct: shRNA TRCN0000180800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152441.1-734s1c1
DNA Barcode:
GCCAGAAGCTCACAGATCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXL14 (144699)
Gene Description:
F-box and leucine rich repeat protein 14
Transcript:
RefSeq NM_152441.1 (NON-CURRENT)
Match location:
Position 734 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000180800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAAGCTCACAGATCTTT
Hairpin Sequence:
5'-CCGG-GCCAGAAGCTCACAGATCTTT-CTCGAG-AAAGATCTGTGAGCTTCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAAGCTCACAGATCTTTCTCGAGAAAGATCTGTGAGCTTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAAGCTCACAGATCTTTCTCGAGAAAGATCTGTGAGCTTCTGGC-3'

Other clones with same target sequence:

(none)